Diverging biological roles among human monocyte subsets in the context of tuberculosis infection

Circulating monocytes (Mo) play an essential role in the host immune response to chronic infections. We previously demonstrated that CD16pos Mo were expanded in Tuberculosis (TB) patients, correlated with disease severity and were refractory to dendritic cell differentiation. Here, we investigated whether human Mo subsets (CD16neg and CD16pos) differed in their ability to influence the early inflammatory response against Mycobacterium tuberculosis (Mtb). We first evaluated the capacity of the Mo subsets to migrate and engage a microbicidal response in vitro. Accordingly, CD16neg Mo were more prone to migrate in response to different mycobacteria-derived gradients, were more resistant to Mtb intracellular growth and produced higher reactive oxygen species than their CD16pos counterpart. To further assess the functional dichotomy among the human Mo subsets, we carried out an in vivo analysis by adapting a hybrid mouse model (SCID/Beige) to transfer each Mo subset, track their migratory fate during Mtb infection, and determine their impact on the host immune response. In Mtb-infected mice, the adoptively transferred CD16neg Mo displayed a higher lung migration index, induced a stronger pulmonary infiltration of murine leukocytes expressing pro- and anti-inflammatory cytokines, and significantly decreased the bacterial burden, in comparison to CD16pos Mo. Collectively, our results indicate that human Mo subsets display divergent biological roles in the context of Mtb infection, a...
Source: Clinical Science - Category: Biomedical Science Authors: Source Type: research

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Source: UN News Centre - Health, Poverty, Food Security - Category: International Medicine & Public Health Source Type: news
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Source: Forbes.com Healthcare News - Category: Pharmaceuticals Authors: Source Type: news
This study determined the BTB prevalence in free-ranging buffaloes in two game reserves and assessed the influence of the prevalence of mycobacterial infections on the performance of a commercial cattle-specific serological assay for BTB (TB ELISA). Buffaloes (n = 997) were tested with the tuberculin skin test and TB ELISA; a subset (n = 119) was tested longitudinally. Culture, PCR and sequencing were used to confirm infection with M. bovis and/or non-tuberculous mycobacteria (NTM). Prevalence of BTB, but not NTM, influenced the TB ELISA performance. Multiple testing did not increase test confid...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
[WHO] Twana Bhembe witnessed the devastating effects of tuberculosis (TB) when her sister died from the disease in 2011. That was before she and her husband were each diagnosed with the disease, a year apart.
Source: AllAfrica News: Tuberculosis - Category: Infectious Diseases Source Type: news
Authors: Mathabire Rucker SC, Cossa L, Harrison RE, Mpunga J, Lobo S, Kisaka Kimupelenge P, Mandar Kol'Ampwe F, Amoros Quiles I, Molfino L, Szumilin E, Telnov O, Ndlovu Z, Huerga H Abstract Background: Determine TB-LAM is a urine-based point-of-care assay for diagnosis of tuberculosis (TB). Objective: To assess the feasibility of using LAM to diagnose TB in adult HIV-positive patients in resource-limited settings. Methods: We performed a multi-centric mixed-methods cross-sectional descriptive study in the Democratic Republic of Congo, Malawi, and Mozambique. We used the study and program monitoring tools to estimat...
Source: Global Health Action - Category: International Medicine & Public Health Tags: Glob Health Action Source Type: research
Authors: Yang S, Wang D, Xu J, Luo F, Wu W, Zhang Z Abstract Objective: To observe the clinical effect of anterior debridement, decompression, bone grafting, and instrumentation for cervical spinal tuberculosis in four hospitals. Materials and Methods: This research retrospectively analyzed 146 patients with cervical spinal tuberculosis who were treated by anterior debridement, decompression, bone grafting, and instrumentation in four institutions between January 2000 and January 2015. There were 68 males and 78 females with an average age of 31.32 ± 11.69 years. All patients received chemotherapy for 18 mon...
Source: Neurological Research - Category: Neurology Tags: Neurol Res Source Type: research
Authors: Pu F, Feng J, Yang L, Zhang L, Xia P Abstract Compared to other diseases, early or atypical spinal tuberculosis (TB) is prone to being misdiagnosed due to the lack of specific clinical manifestations and misleading negative results of various laboratory analyses and imaging examinations. Various methods for diagnosing spinal TB have become research hotspots. Recently, it has been proposed that effective vaccination is a mainstay of long-term policies to combat and control the TB epidemic. However, some new TB vaccines require further evaluation and clinical trials to demonstrate their efficiency and safety...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
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Source: Chinese Medical Sciences Journal - Category: General Medicine Source Type: research
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Source: IDCases - Category: Infectious Diseases Source Type: research
Infectious process even at the initial stage after aerosol infection withMycobacterium tuberculosis induced rapid changes in vaginal microbiota in mice. Rapid decrease in both the quantity and diversity of microbiota was noted, and then, partial recovery of normal flora was observed. Changes in vaginal microbiota was detected as soon as in 3-7 days after lung infection, while inflammatory changes appeared by day 35. At the early stage of infection, no signs of inflammation were observed, neitherM. tuberculosis nor its DNA were detected in mouse genital organs.
Source: Bulletin of Experimental Biology and Medicine - Category: Biology Source Type: research
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