Immunomodulator ‘mushroom beta glucan’ induces Wnt/β catenin signalling and improves wound recovery in tilapia and rat skin: a histopathological study

Abstract
The present study aims to investigate the effects of mushroom beta glucan (MBG) on wound recovery in partial hepatectomy (PH) in Nile tilapia (Oreochromis niloticus) and in rat skin wound healing examination. Following PH, we focussed on the effects on liver repair ability using in vitro and in vivo tests. In vitro, we examined whether the MBG has an impact on liver cell proliferation, mainly through 3‐(4,5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide (MTT) assays and bromodeoxyuridine (BrdU) cell proliferation assay detection method. Results showed that MBG treatment was remarkable in enhancing cell proliferation of hepatocytes and in maintaining the cellular viability. Immunohistochemical staining to analyse Wnt/β‐catenin signalling also showed that MBG has the effect of promoting cell proliferation of liver tissues after PH surgery.

Source: International Wound Journal - April 1, 2015 Category: Surgery Authors: Chien‐Mei Hsiao, Yu‐Sheng Wu, Fan‐Hua Nan, Shih‐Ling Huang, Lynette Chen, Shiu‐Nan Chen Tags: ORIGINAL ARTICLE Source Type: research