Role of gamma radiation on functional expression of the voltage-gated potassium channel Kv10.1 and its importance in the radiobiological response

Appl Radiat Isot. 2022 Jun 16;187:110331. doi: 10.1016/j.apradiso.2022.110331. Online ahead of print.ABSTRACTExposure of biological systems to a radiation absorbed dose produces early and late radiogenic responses, such as ion channel modulation, oxidative stress, cell migration enhancement, and metabolic changes that could impact the efficiency of radiotherapy. To understand how radiation modulates ion channels, we irradiated HEK cells stably expressing the human ether à-go-go potassium channel-1 with gamma photons in the dose range of 2-10 Gy (60Co, 0.2 Gy/min) and measured ionic currents generated by the channel. The importance of the Kv10.1 modulation by gamma radiation was studied using cell proliferation. Results showed that a radiation-absorbed dose of 4 Gy significantly reduced the Kv10.1-evoked currents by depolarizing pulses between -100 mV and +50 mV. Additionally, the expression of Kv10.1 positively modulates HEK293 proliferation and, certainly, prevents the effect of gamma radiation on this phenomenon. Results allow concluding that the modulation of the functional expression of the Kv10.1 channel, induced by gamma radiation, leads to the expression of a radioresistant phenotype in Kv10.1 expressing cells.PMID:35764005 | DOI:10.1016/j.apradiso.2022.110331

Source: Applied Radiation and Isotopes - June 28, 2022 Category: Radiology Authors: Enoch Luis C ésar Oliver Lara Figueroa Mar ía Luisa Durán Pastén Erika Patricia Azor ín Vega Source Type: research