Characterization of Some Microorganisms from Human Stool Samples and Determination of Their Effects on CT26 Colorectal Carcinoma Cell Line
Curr Microbiol. 2022 Jun 15;79(8):225. doi: 10.1007/s00284-022-02915-4.ABSTRACTThe present study aimed to isolate and identify the potential probiotic, pathobiont, and pathogenic microorganisms in the stool samples of 12 healthy individuals and evaluate their in vitro effects on cancer formation. A total of 83 strains were isolated from the stool samples and identified using MALDI-Biotyper. Fourteen of the isolates were identified as Candida spp., three isolates were identified as Cryptococcus neoformans, 55 isolates were identified as lactic acid bacteria, and the remaining isolates belonged to different 11 bacterial genera. Important microbial properties for cancer prevention and some probiotic properties were examined. All strains maintained their viability under acidic conditions and in media containing bile salts. Of the bacterial strains, 62.5% were resistant to ampicillin, chloramphenicol, gentamicin, erythromycin, kanamycin, penicillin, streptomycin, tetracycline, and vancomycin. All yeast strains were resistant to ketoconazole and susceptible to nystatin. The susceptibility of the strains to fluconazole, voriconazole, amphotericin B, and itraconazole varied. Fifty-nine percent of the strains produced EPS and 21.7% showed proteolytic activity (PA). Of the strains, 15.7% both produced exopolysaccharides (EPS) and had PA. The antioxidant activity (AOA) varied depending on the strains. The pathobiont and pathogenic microorganisms promoted tumor formation, while potential...
Source: Current Microbiology - Category: Microbiology Authors: M ünevver Kahraman Aynur G ül Karahan Mustafa Ender Terzio ğlu Source Type: research
More News: Abelcet | Antidoxidants | Bile | Cancer | Cancer & Oncology | Candida | Carcinoma | Colorectal Cancer | Erythromycin | Fluconazole | Gastroenterology | Ketoconazole | Microbiology | Nystatin | Penicillin | Probiotics | Study | Tetracycline