Leucoma salicis nucleopolyhedrovirus (LesaNPV) genome sequence shed new light on the origin of the Alphabaculovirus orpseudotsugatae species
In this study, we have established the relationship between LesaNPV and other closely related alphabaculoviruses. Environmental samples of late instar of white satin moth collected in Poland infected with baculovirus have been homogenized, polyhedra were purified and subjected to scanning and transmission electron microscopy. Viral DNA was sequenced using the Illumina platform and the whole-genome sequence was established by de novo assembly of paired reads. Genome annotation and phylogenetic analyses were performed with the use of bioinformatics tools. The genome of LesaNPV is 132 549 bp long with 154 ORFs and 54.9% GC...
Source: Virus Genes - April 9, 2024 Category: Genetics & Stem Cells Source Type: research
Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave
In conclusion, the lytic phage D6 identified in this study belongs to a novel phage within theCaudoviricetes class and has potential for use in biological control of plant diseases. (Source: Virus Genes)
Source: Virus Genes - April 9, 2024 Category: Genetics & Stem Cells Source Type: research
Analysis of SARS-CoV-2 omicron mutations that emerged during long-term replication in a lung cancer xenograft mouse model
AbstractSARS-CoV-2 Omicron has the largest number of mutations among all the known SARS-CoV-2 variants. The presence of these mutations might explain why Omicron is more infectious and vaccines have lower efficacy to Omicron than other variants, despite lower virulence of Omicron. We recently established a long-term in vivo replication model by infecting Calu-3 xenograft tumors in immunodeficient mice with parental SARS-CoV-2 and found that various mutations occurred majorly in the spike protein during extended replication. To investigate whether there are differences in the spectrum and frequency of mutations between pare...
Source: Virus Genes - April 8, 2024 Category: Genetics & Stem Cells Source Type: research
Potentials as biomarker and therapeutic target of upregulated long non-coding RNA HLA-F antisense RNA 1 in hepatitis B virus-associated hepatocellular carcinoma
This study aimed to identify the clinical and functional roles of long non-coding RNA HLA-F antisense RNA 1 (HLA-F-AS1) in hepatitis B virus (HBV)-hepatocellular carcinoma (HCC). A total of 121 HBV-HCC, 81 chronic hepatitis B (CHB), and 85 normal liver tissues were evaluated in this study. Real-time quantitative PCR assay was used to evaluate the RNA expression levels. Performance in diagnosis was compared between alpha fetoprotein (AFP) and HLA-F-AS1 using Receiver Operating Characteristic (ROC) curves. Performance in post-hepatectomy prognosis with high or low HLA-F-AS1 was compared using Kaplan –Meier curves. Multi-va...
Source: Virus Genes - April 3, 2024 Category: Genetics & Stem Cells Source Type: research
Donkey-like kirkovirus is associated with diarrhea in piglets
In this study, donkey-like kirV was detected in rectal swabs of piglets with diarrhea, and the positive rate was found to be 100% (149/149). However, this virus was detected in only one of 261 clinically healthy piglets, which su ggested a strong relationship between the kirV and the diarrheic disease. We obtained the whole-genome sequences of three kirVs (Cj-D5, Cj-D32, and Cj-D43), with a length of 3750 nucleotides (nt) and sharing 99.9% nt identity with donkey kirVs. Furthermore, the three viruses shared 88.5–100% and 2 3–51% of the Rep protein sequence, similar to available reference strains ofKirkoviridae andCirco...
Source: Virus Genes - March 25, 2024 Category: Genetics & Stem Cells Source Type: research
Isolation and whole-genome sequence analysis of Balagodu virus in Japan
AbstractWhole-genome sequencing of a virus isolated fromCulicoides biting midges in southern Japan in 2020 revealed that it is a strain of Balagodu virus (BLGV; genusOrthobunyavirus; familyPeribunyaviridae; orderBunyavirales). A solitary instance of BLGV isolation occurred in India in 1963. All assembled segments comprise complete protein-coding sequences that are similar to those of other orthobunyaviruses. The consensus 3'- and 5'-terminal sequences of orthobunyaviruses ’ genomic RNAs are also conserved in the Japanese BLGV strain. Here, we update the geographic distribution of BLGV and provide its complete sequence, c...
Source: Virus Genes - March 16, 2024 Category: Genetics & Stem Cells Source Type: research
Ovine adenoviruses infecting sheep and goats in T ürkiye: detection and molecular characterization of three different types
In conclusion, the detection of AdVs in lung and intestinal tissues of small ruminants in this study suggests that these viruses may have contributed to the disease and/or predisposed to other agents. (Source: Virus Genes)
Source: Virus Genes - March 15, 2024 Category: Genetics & Stem Cells Source Type: research
MiR-339-5p inhibits replication of porcine reproductive and respiratory syndrome virus by targeting viral gene regions
In conclusion, miR-339-5p can target the specific PRRSV gene areas and prevent PRRSV replication, offering fresh perspectives for the creation of medications that co mbat the PRRSV infection. (Source: Virus Genes)
Source: Virus Genes - February 18, 2024 Category: Genetics & Stem Cells Source Type: research
Molecular analysis of the env, LTR, and pX regions of bovine leukemia virus in dairy cattle of T ürkiye
This study reports the molecular analysis of the LTR and pX region of BLVs in Türkiye for the first time. (Source: Virus Genes)
Source: Virus Genes - February 14, 2024 Category: Genetics & Stem Cells Source Type: research
Do HBsAg subdeterminants matter for vaccination against hepatitis B?
(Source: Virus Genes)
Source: Virus Genes - February 14, 2024 Category: Genetics & Stem Cells Source Type: research
Characterization of a breakthrough vaccine escape strain associated with overt hepatitis B virus infection
AbstractHepatitis B virus (HBV) vaccine is composed of the purified hepatitis B surface antigen (HBsAg) that is produced by recombinant DNA technology. The neutralizing antibodies induced by vaccination target mainly the “a” determinant, aa124-147, of the outer viral envelope (HBsAg). In the present work, we demonstrate a case study for vaccinated patient that is infected with a vaccine escape HBV strain (Eg200). Characterization of the isolate Eg200 showed that it belongs to the genotype D and an uncommon sub-genotype in Egypt; D9. The DNA sequence encoding HBsAg was sequenced. Mutational analysis of the HBsAg showe...
Source: Virus Genes - February 13, 2024 Category: Genetics & Stem Cells Source Type: research
Isolation and molecular characterization of lumpy skin disease virus from Tamil Nadu, India during the outbreaks from 2020 to 2022
AbstractLumpy skin disease (LSD) caused by LSD virus is a WOAH notifiable, high-impact, transboundary poxviral disease of bovines. The first official report of LSDV in India is from Odisha state during August 2019. Since then, cases have been reported from many states including Tamil Nadu, a Southern state of India. The present study deals with isolation and molecular characterization of LSDV from Tamil Nadu during the period August 2020 to July 2022. LSDV was isolated in embryonated chicken eggs (ECE) and BHK 21 cells and was characterized based onP32, RPO30, andGPCR genes. The phylogenetic analysis revealed that Tamil Na...
Source: Virus Genes - February 12, 2024 Category: Genetics & Stem Cells Source Type: research
Guinea pig herpes like virus is a gamma herpesvirus
AbstractGuinea Pig Herpes-Like Virus (GPHLV) is a virus isolated from leukemic guinea pigs with herpes virus-like morphology described by Hsiung and Kaplow in 1969. GPHLV transformed embryonic cells from Syrian hamsters or rats, which were tumorigenic in adult animals. Herein, we present the genomic sequence of GPHLV strain LK40 as a reference for future molecular analysis. GPHLV has a broad host tropism and replicates efficiently in Guinea pig, Cat, and Green African Monkey-derived cell lines. GPHLV has a GC content of 35.45%. The genome is predicted to encode at least 75 open-reading frames (ORFs) with 84% (63 ORFs) shar...
Source: Virus Genes - February 10, 2024 Category: Genetics & Stem Cells Source Type: research
Correction: Molecular characterization of a novel variant of infectious bronchitis virus from field outbreaks in backyard chicken population of North East India
(Source: Virus Genes)
Source: Virus Genes - February 1, 2024 Category: Genetics & Stem Cells Source Type: research
Comparison of HVT-ND recombinant and convection-based Newcastle disease vaccination programs in the protection against the genotype VII NDV challenges: an experimental study
In this study, a commercial vector vaccine (HVT-NDV) was evaluated compared to the conventional vaccination strategy against Iranian genotype VII. This experiment showed that the group receiving the conventional vaccination strategy had higher antibodies, fewer clinical signs, and lower viral loads in tracheal swabs and feces. Also, two vaccine groups showed significant difference, which could have resulted from two extra vaccine doses in the conventional group. However, except for antibody levels in commercial chickens in the Iran new-generation vaccine, this difference was minor. Further, both groups showed 100% protecti...
Source: Virus Genes - January 30, 2024 Category: Genetics & Stem Cells Source Type: research
