Editorial Comments: looking for some innovative diagnostic or developmental research studies that might influence our laboratory and clinical decision-making
For this issue, I have invited two leading experts to share the outcome of their innovative ongoing studies on the advancement of in vivo assessment of RBC and platelet function testing in specialized emergency cases. Prof. Jason Acker ’s team reports on two interrelated topics that are exceedingly relevant to modern Transfusion Medicine. The first manuscript is aimed at the influence of RBC quality in red cell concentrates on vascular redox homeostasis and associated redox signalling measures which are novel in vivo quality mea surements of red cell concentrates and reflect future directions in post-transfusion redox an...
Source: Transfusion and Apheresis Science - April 18, 2024 Category: Hematology Authors: Jerard Seghatchthiian Source Type: research
Graft collection by apheresis and graft composition
Ever since Hematopoietic Stem Cells (HSCs) were first identified in blood [1] there has been great interest in developing alternative methods to bone marrow harvesting under general anesthesia. In particular, the introduction of techniques for extensive collection of blood cells by leukapheresis and the knowledge of factors affecting stem cell concentration in blood, such as a great stem cell output after chemotherapy [2], growth factor administration [3] [Granulocyte Colony Stimulating Factor (G-CSF), Plerixafor] or their combination, has allowed, nowadays, the replacement of bone marrow harvests with blood stem cell coll...
Source: Transfusion and Apheresis Science - April 17, 2024 Category: Hematology Authors: Luca Pierelli, Anna Capalbo Source Type: research
Impact of ABO blood group on mortality in trauma patients: A systematic review
Hemorrhage is a significant cause of death in trauma patients. There is evidence that individuals with blood group O have higher rates of non-traumatic haemorrhage. It has been suggested that blood group O may be associated with higher mortality in trauma, however existing evidence is limited and conflicting. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 17, 2024 Category: Hematology Authors: Andrew Follows, Antonia Round Source Type: research
Mobilization and collection of cd34+ cells in patients with globin disorders: providing the starting material to manufacturers of autologous gene therapies
Globin gene disorders are a group of inherited diseases that result in altered production, structure or function of hemoglobin. The prevalence of these chronic ailments is significantly related to genetic and ethnic backgrounds, with a high fraction of patients living in low- to medium-income countries [1]; however, and as a result of migrations over centuries, a smaller fraction of patients reside in high-income countries. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 17, 2024 Category: Hematology Authors: Christian Chabannon, Sandrine Visentin, Ang éla Granata, Isabelle Thuret Source Type: research
Advancing In Vivo Assessment of Red Blood Cell Transfusions: A Call for Radiation-Free Methods in Transfusion Medicine
This article reviews existing in vivo techniques, like chromium-51 labelling and biotinylation, for determining the circulation and survival of RBCs, and advocates for a move to radiation-free methods. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 17, 2024 Category: Hematology Authors: Mahsa Yazdanbakhsh, Jason P. Acker Source Type: research
A Perspective on the Exogenous Redox Regulation of Transfused RBCs Subject to the Storage Lesion
Granted with a potent ability to interact with and tolerate oxidative stressors, RBCs scavenge most reactive oxygen and nitrogen species (RONS) generated in circulation. This essential non-canonical function, however, renders RBCs susceptible to damage when vascular RONS are generated in excess, making vascular redox imbalance a common etiology of anemia, and thus a common indication for transfusion. This accentuates the relevance of impairments in redox metabolism during hypothermic storage, as the exposure to chronic oxidative stressors upon transfusion could be exceedingly deleterious to stored RBCs. (Source: Transfusio...
Source: Transfusion and Apheresis Science - April 17, 2024 Category: Hematology Authors: Nishaka William, Jason P. Acker Source Type: research
Platelet function testing: update on determinant variables and permissive windows using a platelet-count-based device
While there are various aspects of platelet biology that can be studied in the lab (i.e. adhesion, degranulation, integrin activation), the master test for platelet function is that which gives a measure of the platelet aggregation capacity upon stimulation with an agonist. Platelet function testing is necessary for the diagnosis of platelet disorders and the monitoring of patients receiving anti-platelet treatments. Furthermore, it becomes relevant in the quality control of platelet concentrates for transfusion purposes, especially considering the global concern about long term storage, other forms of storage (i.e. (Sourc...
Source: Transfusion and Apheresis Science - April 17, 2024 Category: Hematology Authors: Patricia Villar, Sof ía Carreño, Sara Moro, Inés Díez Galindo, Ángel Bernardo, Laura Gutiérrez Source Type: research
How to manage poor mobilisers
Autologous hematopoietic progenitor cell transplantation (ASCT) has been used for more than five decades to treat malignant and non-malignant diseases. Successful engraftment after high-dose chemotherapy relies on the ability to collect sufficient CD34+ hematopoietic progenitor cells (HPCs), typically from peripheral blood after mobilization. Commonly, either granulocyte colony-stimulating factor (G-CSF) alone as a single agent (i.e. steady-state mobilization) or G-CSF after chemotherapy is administered to collect adequate numbers of HPCs (minimum ≥2 × 106 CD34+ cells/kg for one ASCT; optimal up to 5 × 106 CD34+ cells/...
Source: Transfusion and Apheresis Science - April 17, 2024 Category: Hematology Authors: Nina Worel Source Type: research
Prevalence and Factors Influencing Anemia Recovery after Intensive Care
This study aimed to investigate the prevalence of anemia following ICU discharge and factors affecting recovery from anemia. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 15, 2024 Category: Hematology Authors: Kyoung Won Yoon, Sungjoo Park, Chi-Min Park Source Type: research
Impact of Cell Salvage on Hematocrit and Post-Partum Anemia in Low Hemorrhage Risk Elective Cesarean Delivery
Postpartum anemia is a significant contributor to peripartum morbidity. The utilization of cell salvage in low risk cases and its impact on postpartum anemia has not been investigated. We therefore aimed to examine the impact of autologous blood transfusion/cell salvage in routine cesarean delivery on postoperative hematocrit and anemia. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 15, 2024 Category: Hematology Authors: Daniel Katz, Annalin Griffel, Sarah Granozio, Gary Koenig, Hung-Mo Lin Source Type: research
Editorial Board
(Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 1, 2024 Category: Hematology Source Type: research
Prevention of Potential Delayed Hemolytic Transfusion Reaction in Two Sickle Cell Patients Using Intravenous Immunoglobulins and Steroids Before and After Red Blood Cell Exchange with Antigen Positive Units and Review Literature
Emergent Red Blood Cell (RBC) exchange is indicated in sickle cell disease (SCD) patients with severe acute chest syndrome. However, fully matched RBC units may not be available for patients with multiple RBC antibodies. Intravenous immunoglobulin (IVIG) and steroids were reported for preventing potential delayed hemolytic transfusion reaction (HTR) in simple transfusion of antigen-positive RBCs. We investigated the efficacy and safety of IVIG and steroids in two SCD patients presented with acute chest syndrome receiving RBC exchange with multiple incompatible units. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 1, 2024 Category: Hematology Authors: Janna Shold, Amitava Dasgupta, Zhan Ye Source Type: research
Molecular and Clinical Profile of Rare Bleeding Disorders: A Single-center Retrospective Study
Due to their low frequency, there is little information on the molecular pathologies of rare bleeding disorders (RBD). Therefore, this study aimed to analyze the molecular and clinical profiles of patients with RBD. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - April 1, 2024 Category: Hematology Authors: Arash Ahmadfard Moghadam, Amir Reza Manafzadeh, Mohammad Reza Nikoonia, Khadijeh Dajliry, Farahnaz Ramezan, Shadi Tabibian Source Type: research
Impact of therapeutic plasma exchange on intact protein S, apolipoproteins, and thrombin generation
Therapeutic plasma exchange (TPE), with solvent/detergent (S/D)-treated plasma as replacement fluid, is an extracorporeal blood purification technique with major impact on both coagulation and lipids. Our previous in vitro study showed that S/D-plasma enhances thrombin generation by lowering intact protein S (PS) levels. Aims: To evaluate the impact of altered lipid balance on coagulation phenotype during heparin-anticoagulated TPE with S/D-plasma, and to investigate whether the lowered intact PS levels with concomitant procoagulant phenotype, are recapitulated in vivo. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - March 27, 2024 Category: Hematology Authors: Hanna H Pitk änen, Mikko Haapio, Mika Saarela, Marja-Riitta Taskinen, Herm-Jan Brinkman, Riitta Lassila Source Type: research
Utility of Hemoglobin Electrophoresis to Distinguish Between Severe Delayed Hemolytic Transfusion Reaction Versus Hyperhemolysis Syndrome
Delayed hemolytic transfusion reaction (DHTR) and hyperhemolysis syndrome (HHS) are both complications of red blood cell transfusions in patients with sickle cell disease.Clinically, both present with hemolysis and can be difficult to differentiate. Hemoglobin electrophoresis may aid in the diagnosis. Herein we describe a case in which a patient with hemoglobin SC disease presented with features of severe hemolysis several days after initiation of red blood cell exchange. Increase in reticulocyte count and complete absence of hemoglobin A on electrophoresis during this event supported the diagnosis of severe DHTR, indicati...
Source: Transfusion and Apheresis Science - March 27, 2024 Category: Hematology Authors: Robert Lukin, Jennie Y. Law, Parvez M. Lokhandwala Source Type: research
