Editorial Board
(Source: Theriogenology)
Source: Theriogenology - June 13, 2018 Category: Genetics & Stem Cells Source Type: research
Assessment of Semen Quality and Fertility in Young Growing Beef Bulls Exposed to Ergot Alkaloids
The objective of this study was to evaluate semen quality and fertility in beef bulls grazing the ergot alkaloid (EA) producing tall fescue cultivar, Kentucky 31 (KY31), compared to a novel endophyte (NE) cultivar lacking EA. Two studies were conducted over a 3-year period. In studies 1 (n = 10; ages ≥ 24 mo) and 2 (n = 53 over two years; ages 12-16 mo), Angus (AN) bulls were stratified by body weight (BW), body condition score (BCS), and scrotal circumference (SC), and then allotted to graze either KY31 or NE for 56 days. (Source: Theriogenology)
Source: Theriogenology - June 12, 2018 Category: Genetics & Stem Cells Authors: C.R. Burnett, S.L. Pratt, N.M. Long, G.S. Sell, F.N. Schrick Source Type: research
Effect of Aminopeptidase N on functions and fertility of mouse spermatozoa in vitro
Aminopeptidase N (APN) is defined as a multifunctional enzyme, which regulate cellular physiology of a wide variety of cells in human. Earlier studies reported that mammalian semen shares this common enzyme as a major protein of seminal plasma that has correlation with male fertility, while the regulatory mechanisms of APN in spermatozoa are still far from being well understood. Present study was designed to investigate the role of APN in biological and chemical functions of spermatozoa using an in vitro antagonistic approach. (Source: Theriogenology)
Source: Theriogenology - June 11, 2018 Category: Genetics & Stem Cells Authors: Amena Khatun, Kyu-Ho Kang, Do-Yeal Ryu, Md Saidur Rahman, Woo-Sung Kwon, Myung-Geol Pang Source Type: research
Sublethal sperm freezing damage: Manifestations and solutions
Semen cryopreservation is an important tool for artificial breeding, species conservation and human reproductive medicine. However, sublethal freezing damage remains an important limitation of the cryopreservation process, inevitably leading to reduced fertility in vivo. This review explores several facets of sublethal freezing damage, touching on cryocapacitation, the generation of reactive oxygen species and alterations to sperm proteins, lipids and sugars. The effects of sublethal freezing damage on sperm performance in vivo are also discussed, examining fertile lifespan and interaction with female reproductive tract im...
Source: Theriogenology - June 11, 2018 Category: Genetics & Stem Cells Authors: Taylor Pini, Tamara Leahy, Simon P. de Graaf Source Type: research
The toxic effect of aflatoxin B1 on early porcine embryonic development Running head: The effect of AFB1 on porcine embryos
Aflatoxin B1 (AFB1) is a type of mycotoxin produced by the fungi Aspergillus flavus and Aspergillus parasiticus. AFB1 is considered as the most toxic mycotoxin owing to its toxic effect on health. In the present study, the toxic effect of AFB1 on early porcine embryonic development and its possible mechanism were investigated. Blastocyst formation was impaired with treatment of 1 nM AFB1 compared with control, 0.01, 0.1 group (40.13 ± 2.10%, 28.21 ± 1.62%, 32.34 ± 2.07% vs 19.01 ± 1.06%). Further study showed that the presence of AFB1 induced the generation of reactive oxygen species (ROS). (Source: Theriogenology)
Source: Theriogenology - June 8, 2018 Category: Genetics & Stem Cells Authors: Kyung-Tae Shin, Jing Guo, Ying-Jie Niu, Xiang-Shun Cui Source Type: research
Reproductive performance of Bos indicus beef cows treated with different doses of equine chorionic gonadotropin at the end of a progesterone-estrogen based protocol for fixed-time artificial insemination
Two experiments were performed to evaluate the reproductive performance of zebu beef cows treated with different doses of eCG at the end of a progesterone (P4)/estrogen based protocol for timed artificial insemination (TAI). In Experiment 1, suckling Bos indicus Nelore cows (n=261) received, on day 0, a progesterone (P4) intravaginal device (PD) and an injection of 1 mg estradiol benzoate (EB). On day 8, the PD was removed, 500 μg of cloprostenol was injected, and cows were assigned to one of the following groups: Control (no treatment), 300 (300 IU of eCG), 600 (600 IU of eCG), and 900 (900 IU of eCG). (Source: Theriogenology)
Source: Theriogenology - June 7, 2018 Category: Genetics & Stem Cells Authors: Rafael Herrera Alvarez, Guilherme Pugliesi, Fabio Luis Nogueira Natal, Cecilia Constantino Rocha, Gilmar Arantes Ataide J únior, Alfredo José Ferreira Melo, Ivani Posar Otzuk, Claudio Alvarenga de Oliveira, Patrice Humblot Source Type: research
Effect of RFRP-3, the mammalian ortholog of GnIH, on the epididymis of male rats
RFamide-related peptide-3 (RFRP-3) and its receptor (GPR147) play an important role in reproduction regulation in mammals. To understand the role of RFRP-3 in male reproductive function of epididymis, we first investigated the expression changes in RFRP-3 and its receptor at different stages of development, that is, postnatal day 20 (P20), 40 (P40), 60 (P60) and 80 (P80). Our results showed that fluctuations in the expression of GnIH and GPR147 during postnatal development occurred, and the highest epididymal GnIH and GPR147 expression were both detected in P60. (Source: Theriogenology)
Source: Theriogenology - June 5, 2018 Category: Genetics & Stem Cells Authors: Xiaoye Wang, Genglin Guo, Xin Zhang, Ming Li, Kai Xiao, Chuanhuo Hu, Xun Li Source Type: research
Molecular characterization and expression analysis of strbp in Chinese tongue sole (Cynoglossus semilaevis)
In this study, we have isolated and characterized the strbp gene of Chinese tongue sole, Cynoglossus semilaevis, termed as CS-strbp. The CS-strbp genomic sequence contained fifteen exons and fourteen introns. Its cDNA was 2,655 bp in length, encoding a 666-amino-acid protein with two conserved ds binding motifs. Using quantitative PCR, we found that CS-strbp mRNA exhibited sex-biased and tissue-specific distribution, predominantly expressed in the fertile male testis, though the expression levels varied throughout different developmental stages. (Source: Theriogenology)
Source: Theriogenology - June 5, 2018 Category: Genetics & Stem Cells Authors: Liang Meng, Wenteng Xu, Ying Zhu, Ning Zhang, Changwei Shao, Yang Liu, Songlin Chen Source Type: research
Effect of egg yolk plasma and soybean lecithin on rooster frozen-thawed sperm quality and fertility
This experiment was conducted to study the effects of egg yolk plasma (10%, 15% and 20%), soybean lecithin (0.5%, 1% and 1.5%) and whole egg yolk (WEY) (control) on post-thawed sperm quality, hatchability and fertility outcomes. In experiment 1, sperm motility, abnormalities, membrane integrity, viability, apoptosis status, mitochondrial activity were studied following freeze-thawing. The best quality of frozen-thawed rooster sperm was chosen to be used for the assessment of the hatchability and fertility rate in experiment 2. (Source: Theriogenology)
Source: Theriogenology - June 2, 2018 Category: Genetics & Stem Cells Authors: Mahdieh Mehdipour, Hossein Daghigh Kia, Gholamali Moghaddam, Hamed Hamishehkar Source Type: research
Editorial Board
(Source: Theriogenology)
Source: Theriogenology - June 2, 2018 Category: Genetics & Stem Cells Source Type: research
Postponement of canine puberty by neonatal administration of a long term release GnRH superagonist
The objective of this study was to assess the efficiency and clinical safety of postnatal administration of a GnRH agonist on canine puberty postponement. Sexual steroids and histological gonadal changes were also described. Twenty-four littermate puppies were randomly assigned to: Deslorelin acetate 18.8 mg sc (DESLO; n = 12) or Placebo: sc (PLACE; n = 12) postnatally. The dogs were clinically and endocrinologically followed up until puberty when they were gonadectomized and their gonads histomorphometrically studied. (Source: Theriogenology)
Source: Theriogenology - June 2, 2018 Category: Genetics & Stem Cells Authors: M. Faya, C. Marchetti, M. Priotto, M. Grisol ía, F. D´Francisco, C. Gobello Source Type: research
Roles of the sire and dam quail in egg, yolk, albumen, and shell weight alterations due to the parthenogenetic trait
Infertile eggs from Chinese Painted quail exhibit parthenogenesis, and the 1st egg in a clutch sequence is more likely to develop a parthenogenetic embryo. Also, hens that exhibit parthenogenesis have shorter clutches and heavier egg weights. These larger eggs may be a result of the egg residing in the oviduct longer, allowing more time for the parthenote to develop. However, it is unknown which components of the egg are altered due to the parthenogenetic trait or the role of dams and sires from the parthenogenetic line of quails in these alterations. (Source: Theriogenology)
Source: Theriogenology - June 1, 2018 Category: Genetics & Stem Cells Authors: R. Ramachandran, M. Nascimento dos Santos, A.J.T. Kawaoku, C.D. McDaniel Source Type: research
Parental Sex Effect of Parthenogenesis on Progeny Production and Performance of Chinese Painted Quail (Coturnix Chinensis)
Embryonic development of an unfertilized egg, parthenogenesis, is known to occur in Chinese Painted quail. However, selection for parthenogenesis in both the dam and sire leads to a reduction in hatchability following mating. Therefore, the objective of this study was to determine if selection for parthenogenesis in the dam, sire, or both also impacts their progeny performance. There were 2 lines of birds used in this trial: 1 line selected for parthenogenesis and 1 line not selected for parthenogenesis (control) yielding breeding pairs as follows: control dams + control sires (CC), control dams + parthenogenetic sires (CP...
Source: Theriogenology - June 1, 2018 Category: Genetics & Stem Cells Authors: R. Ramachandran, M. Nascimento dos Santos, H.M. Parker, C.D. McDaniel Source Type: research
Influences of intrauterine semen administration on regulatory T lymphocytes in the oestrous mare (Equus caballus)
In the mare, early pregnancy loss is common, but involvement of the maternal immune system in the pathogenesis of this condition has not been investigated in detail so far. In the present study, we assessed effects of exposure of the endometrium to semen or seminal plasma in oestrous mares on the response of regulatory T lymphocytes (Tregs) in the peripheral circulation as well as in the endometrium. Raw semen, seminal plasma or PBS (control) were introduced into the uterus of oestrous mares (n=12). (Source: Theriogenology)
Source: Theriogenology - June 1, 2018 Category: Genetics & Stem Cells Authors: Constanze Hartmann, Wilhelm Gerner, Ingrid Walter, Armin Saalm üller, Christine Aurich Source Type: research
Treatment with acetyl-L-carnitine during in vitro maturation of buffalo oocytes improves oocyte quality and subsequent embryonic development
Oocyte quality is one of the important factors in female fertility, in vitro maturation (IVM), and subsequent embryonic development. In the present study, we assessed whether acetyl-L-carnitine (ALC) supplementation during in vitro maturation of buffalo oocytes could improve oocyte quality and subsequent embryonic development. To determine the optimal level of ALC supplementation, we matured cumulus-oocyte complexes in maturation medium supplemented with 0, 2.5, and 5 mM ALC. The oocytes with a polar body were selected for parthenogenetic activation (PA) and in vitro fertilization (IVF). (Source: Theriogenology)
Source: Theriogenology - June 1, 2018 Category: Genetics & Stem Cells Authors: Hui-Yan Xu, Xiao-Gan Yang, Sheng-Sheng Lu, Xing-Wei Liang, Yang-Qing Lu, Ming Zhang, Ke-Huan Lu Source Type: research
