A ratiometric electrochemical biosensor based on ARGET ATRP for detection of HER2 gene
Talanta. 2024 Apr 22;275:126130. doi: 10.1016/j.talanta.2024.126130. Online ahead of print.ABSTRACTHuman epidermal growth factor receptor 2 (HER2), a common proto-oncogene, is overexpressed in a subset of breast cancer patients. It is essential to track HER2 expression for early breast cancer diagnosis. Herein, a ratiometric electrochemical biosensor for detection of HER2 based on activators generated by electron transfer for atom transfer radical polymerisation (AGET ATRP) and hairpin DNA was developed. Specifically, hairpin DNA was first self-assembled on the gold electrode by Au-S bond. Upon capturing HER2, the stem-loo...
Source: Talanta - April 23, 2024 Category: Chemistry Authors: Aozi Feng Li Li Ningxia He Daoxiang Li Danna Zheng Yanju Liu Huaixia Yang Source Type: research
Controlled silver electrodeposition on gold nanoparticle antibody tags for ultrasensitive prostate specific antigen sensing using electrochemical and optical smartphone detection
Talanta. 2024 Apr 16;275:126095. doi: 10.1016/j.talanta.2024.126095. Online ahead of print.ABSTRACTOne of the current challenges in medicine is to achieve a rapid and unequivocal detection and quantification of extremely low levels of disease biomarkers in complex biological samples. Here, we present the development and analytical evaluation of a low-cost smartphone-based system designed for ultrasensitive detection of the prostate-specific antigen (PSA) using two detection alternatives: electrochemical or optical, by coupling the smartphone with a portable potentiostat or magnifying lenses. An antibody tagged with gold na...
Source: Talanta - April 23, 2024 Category: Chemistry Authors: Guillermo Redondo-Fern ández Laura Cid-Barrio Mar ía T Fernández-Argüelles Alfredo de la Escosura-Mu ñiz Ana Soldado Jos é M Costa-Fernández Source Type: research
The combination of deep learning and pseudo-MS image improves the applicability of metabolomics to congenital heart defect prenatal screening
In conclusion, the CNN model based on pseudo-MS images holds promise for real-world and clinical applications due to its better repeatability.PMID:38648686 | DOI:10.1016/j.talanta.2024.126109 (Source: Talanta)
Source: Talanta - April 22, 2024 Category: Chemistry Authors: Borui Sun Yiwei Fang Hui Yang Fan Meng Chao He Yun Zhao Kai Zhao Huiping Zhang Source Type: research
Evaluating smartphone-based optical readouts for immunoassays in human and veterinary healthcare: A comparative study
This study uses high-resolution cameras in mobile devices to capture and process bioassay images, enabling the quantification of diverse biomarkers across a range of diagnostic tests conducted on 96-well microplates. The study evaluates the effectiveness of this technology through protein quantification techniques and immunoassays that generate colorimetric responses at specific wavelengths. It includes the assessment of bicinchoninic acid and Bradford protein quantification methods, alongside a conventional immunoassay for detecting mare antibodies in colostrum to monitor foal immunodeficiencies. Further application invol...
Source: Talanta - April 22, 2024 Category: Chemistry Authors: Melania Mesas G ómez Esther Juli án Lara Armengou Maria Isabel Pividori Source Type: research
Detection of paracetamol by a montmorillonite-modified carbon paste sensor: A study combining MC simulation, DFT computation and electrochemical investigations
This study aims to develop a suitable electrochemical electrode through the incorporation of potassium montmorillonite (MMTK10)clay into the carbon matrix for the direct and sensitive determination of paracetamol (PAR) in pharmaceutical formulations. Electrochemical characterization of the electrodes involves the use of techniques such as cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). The results reveal that the voltammetric response of PAR is linear over a wide concentration range (1.0-15 μM), with a low detection limit of 0.46 μM. Analytically, PAR recov...
Source: Talanta - April 21, 2024 Category: Chemistry Authors: Mohamed Achache Ghizlane Elouilali Idrissi Anas Chraka Nordin Ben Seddik Khalid Draoui Dounia Bouchta Choukairi Mohamed Source Type: research
A zirconium metal-organic framework functionalized with a S/N containing carboxylic acid (MOF-808(Zr)-Tz) as an efficient sorbent for the ultrafast and selective dispersive solid phase micro extraction of chromium, silver, and rhodium in water samples
Talanta. 2024 Apr 16;274:126094. doi: 10.1016/j.talanta.2024.126094. Online ahead of print.ABSTRACTMetal-organic frameworks (MOFs) are good adsorbents for targeted chemicals with their adjustable properties. Herein, we prepared a zirconium based MOF (MOF-808(Zr)) and functionalized it employing 2-mercapto-4-methyl-5-thiazolacetic acid (MOF-808(Zr)-Tz). The prepared MOFs were characterized by XRD, FTIR, SEM-EDX, TGA, N2 sorption, zeta potential measurements, and elemental analysis. The surface area of MOF-808(Zr)-Tz was 1348 m2/g. Dispersive solid-phase micro-extraction (D-SPµE) method based on MOF-808(Zr)-Tz was firstly d...
Source: Talanta - April 21, 2024 Category: Chemistry Authors: Şerife Tokalıoğlu Seyed Taeed Hosseini Moghaddam Sel çuk Demir Source Type: research
Gold leaf electrochemical flow cell for determination of iodide in nuclear emergency tablets
Talanta. 2024 Apr 9;275:125963. doi: 10.1016/j.talanta.2024.125963. Online ahead of print.ABSTRACTThis work introduces an innovative gold-leaf flow cell for electrochemical detection in flow injection (FI) analysis. The flow cell incorporates a hammered custom gold leaf electrochemical sensor. Hammered gold leaves consist of pure gold and are readily available in Thailand at affordable prices (approximately $0.085 for a sheet measuring 40 mm × 40 mm). Four sensing devices can be made from a single sheet of this gold leaf, resulting in a production cost of approximately $0.19 per sensor. Each electrochemical sensor has the...
Source: Talanta - April 21, 2024 Category: Chemistry Authors: Kurnia Hidayatulloh Alqibthiyah Paithoon Prasertying Takdanai Techarang Wichayaporn Kamsong Hermin Sulistyarti Kanchana Uraisin Duangjai Nacapricha Source Type: research
Orange carbon dots based smart sensing platforms for rapid, visual, quantitative identification of sodium copper chlorophyllin
Talanta. 2024 Apr 10;275:126090. doi: 10.1016/j.talanta.2024.126090. Online ahead of print.ABSTRACTA highly affordable, sensitive and portable detection platform for the quantitative identification of sodium copper chlorophyllin (SCC) in food and environment is a crucial need. Even though many carbon dots (CDs) based sensors have been developed, few reports on using CDs as optical probes for SCC detection have been published so far. In this paper, orange luminescent CDs (OLCDs) were prepared via solvothermal method, which have high fluorescence quantum yield (27.20 %) and excellent photostability. OLCDs can detect SCC via ...
Source: Talanta - April 20, 2024 Category: Chemistry Authors: Xiao Jin Min Zheng Source Type: research
Sensitivity and stability improvement on slippery surface-aggregated substrate for trace heavy metals detection using NELIBS
Talanta. 2024 Apr 14;275:126001. doi: 10.1016/j.talanta.2024.126001. Online ahead of print.ABSTRACTThe sensitive and stable detection of trace heavy metals in liquid is crucial given its profound impact on various aspects of human life. Currently, nanoparticle-enhanced laser-induced breakdown spectroscopy (NELIBS) with dried droplet method (DDM) is widely applied for heavy metals detection. Nevertheless, the coffee ring effect (CRE) in DDM affects the stability, accuracy, and sensitivity of NELIBS. Here, we developed a slippery surface-aggregated substrate (SS substrate) to suppress the CRE and enrich analytes, and form a ...
Source: Talanta - April 20, 2024 Category: Chemistry Authors: Siyi Xiao Yuanchao Liu Yawen Luo Yuying Zhu Weiliang Wang Junfei Nie Weihua Huang Chen Niu Aojun Gong Lianbo Guo Source Type: research
Recent advances in gold nanoparticles-based biosensors for tuberculosis determination
Talanta. 2024 Apr 12;275:126099. doi: 10.1016/j.talanta.2024.126099. Online ahead of print.ABSTRACTTuberculosis (TB) is one of the major killer diseases affecting lung parenchymal tissues. Mycobacterium tuberculosis (Mtb) is the bacterium that causes it. It most commonly affects the lungs, although it can affect any part of the body, including the stomach, glands, bones, and nervous system. Although anti-mycobacterial drugs are available, it remains a major threat to public health due to the rise of drug-resistant strains, and early and accurate diagnosis is very important. Currently, research science and medical communiti...
Source: Talanta - April 19, 2024 Category: Chemistry Authors: Reza Eivazzadeh-Keihan Zahra Saadatidizaji Mohammad Mahdavi Ali Maleki Mohammad Irani Iman Zare Source Type: research
Fragmentation characteristics-based nontargeted screening method of exogenous chemical residues in animal-derived foods using reversed-phase and hydrophilic interaction liquid chromatography-high-resolution mass spectrometry
In this study, first, fragmentation characteristics of 51 classes of exogenous chemical residues were summarized based on experimental mass spectra of standards in reversed-phase and hydrophilic interaction liquid chromatography-high-resolution mass spectrometry (MS) and mass spectra from the MassBank of North America (MoNA) library. According to the proportion of fragmentation characteristics to the total number of chemical residues in each class, four screening levels were defined to classify 51 classes of chemical residues. Then, a nontargeted screening method was developed based on the fragmentation characteristics. Th...
Source: Talanta - April 19, 2024 Category: Chemistry Authors: Wenying Liang Tiantian Chen Yujie Zhang Xin Lu Xinyu Liu Chunxia Zhao Guowang Xu Source Type: research
Hydrophobic carbon quantum dots with red fluorescence: An optical dual-mode and smartphone imaging sensor for identifying Chinese Baijiu quality
Talanta. 2024 Apr 12;275:126064. doi: 10.1016/j.talanta.2024.126064. Online ahead of print.ABSTRACTChinese Baijiu (Liquor) is a popular alcoholic beverage, and the ethanol content in Baijiu is closely related to its quality; therefore, it is of great significance to explore a facile, sensitive, and rapid method to detect ethanol content in Baijiu. Hydrophobic carbon quantum dots (H-CQDs) with bright red fluorescence (24.14 %) were fabricated by hydrothermal method using o-phenylenediamine, p-aminobenzoic acid, manganese chloride, and hydrochloric acid as reaction precursors. After the introduction of ultrapure water into t...
Source: Talanta - April 19, 2024 Category: Chemistry Authors: Zihan Wang Yang Liu Meiqi Liang Yihong Chen Wenjuan Dong Qin Hu Shengmei Song Shaomin Shuang Chuan Dong Xiaojuan Gong Source Type: research
Molecular engineering of fluorescent dyes for long-term specific visualization of the plasma membrane based on alkyl-chain-regulated cell permeability
Talanta. 2024 Apr 14;275:126105. doi: 10.1016/j.talanta.2024.126105. Online ahead of print.ABSTRACTLong-term visualization of changes in plasma membrane dynamics during important physiological processes can provide intuitive and reliable information in a 4D mode. However, molecular tools that can visualize plasma membranes over extended periods are lacking due to the absence of effective design rules that can specifically track plasma membrane fluorescent dye molecules over time. Using plant plasma membranes as a model, we systematically investigated the effects of different alkyl chain lengths of FMR dye molecules on thei...
Source: Talanta - April 19, 2024 Category: Chemistry Authors: Chuangye Yao Jiaqi Zuo Penglei Wu Jie Liu Junjun Pan Engao Zhu Hui Feng Kewei Zhang Zhaosheng Qian Source Type: research
Smartphone-assisted array discrimination of sulfur-containing compounds and colorimetric-fluorescence dual-mode sensor for detection of 1,4-benzenedithiol based on peroxidase-like nanozyme g-C < sub > 3 < /sub > N < sub > 4 < /sub > @Cu, N-CDs
Talanta. 2024 Apr 16;275:126119. doi: 10.1016/j.talanta.2024.126119. Online ahead of print.ABSTRACTPresent work reported a novel nanozyme g-C3N4@Cu, N-CDs with excellent peroxidase-like activity obtained by loading Cu and N co-doped carbon dots on g-C3N4 (graphitic carbon nitride). g-C3N4@Cu, N-CDs can catalyze H2O2 to generate hydroxyl radical •OH, which oxidizes o-phenylenediamine to 2,3-diaminophenazine, which emits orange fluorescence under ultraviolet light irradiation. The experimental results confirmed that 1,4-benzenedithiol (BDT) could inhibit the peroxidase-like activity of g-C3N4@Cu, N-CDs. Based the principle...
Source: Talanta - April 19, 2024 Category: Chemistry Authors: Linchun Nie Liushan Jiang Shuangying Li Denghao Song Guangyu Dong Lutong Bu Chunmao Chen Qingxiang Zhou Source Type: research
A microfluidic chip platform based on Pt nanozyme and magnetized phage composite probes for dual-mode detecting and imaging pathogenic bacteria viability
Talanta. 2024 Apr 7;275:126067. doi: 10.1016/j.talanta.2024.126067. Online ahead of print.ABSTRACTThe detection of pathogen viability is critically important to evaluate its infectivity. In the study, an integrated microfluidic chip based on dual-mode analytical strategy was developed to rapidly realize detection of bacteria activity (with Salmonella typhimurium, S.T, as a model analyte). Firstly, the composite probes, including deactivated phage modified magnetic beads and nano Pt-antimicrobial peptide (AMP) which can specifically recognize Gram-negative bacteria as nanozyme were prepared. When the composite probes are in...
Source: Talanta - April 19, 2024 Category: Chemistry Authors: Bailu Liu Jingya Cao Binxin Hong Hang You Tianhua Li Zhenzhong Yu Dengfeng Li Baihui Liang Ning Gan Source Type: research
