T-cell acute lymphoblastic leukemia with atypical intracytoplasmic inclusions in the blasts
This study highlights the diagnostic potential of flow cytometry in identifying T-ALL, allowing for a more comprehensive evaluation of diagnostic characteristics. The study demonstrates that flow cytometry can significantly enhance the diagnosis of T-ALL by offering a more accurate and efficient alternative to traditional hematopathological examination. Simultaneous evaluation of a wide range of diagnostic characteristics is made possible by this method, thereby improving our ability to detect and differentiate T-cell disorders like T-ALL. CD7, Cy CD3, CD5, CD13, CD38, CD117, and CD45 are identified as reliable indicators ...
Source: Comparative Clinical Pathology - April 5, 2024 Category: Pathology Source Type: research
Antioxidant and anticholinesterase activity of rutin in aluminum chloride-exposed Drosophila melanogaster
This study investigated the activity of rutin in mitigating Al chloride (AlCl3) toxicity inDrosophila melanogaster. Flies were divided into six groups containing fifty (50) flies each. Group A served as the control; Group B received 40 mM AlCl3; Groups C and D were co-treated with 40 mM AlCl3 + 0.5 mg/kg rutin and 40 mM AlCl3 + 1 mg/kg, respectively; Groups E and F were treated with rutin alone in doses of 0.5 mg/kg and 1 mg/kg, respectively, all through their diet. Negative geotaxis, superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and acetylcholinesterase (AChE) activities were evaluate...
Source: Comparative Clinical Pathology - April 4, 2024 Category: Pathology Source Type: research
Cajanus cajan induces mitochondrial-mediated apoptosis via caspase activation and cytochrome c release
ConclusionsThese findings suggest thatC. cajan-induced mitochondrial-mediated apoptosis in normal rat liver through the induction of mPT and eventual release of cytochrome c which is a prelude to the progression of apoptosis. Hence, certain bioactive components ofC. cajan may be useful in chemoprevention and management of conditions associated with insufficient apoptosis. (Source: Comparative Clinical Pathology)
Source: Comparative Clinical Pathology - March 16, 2024 Category: Pathology Source Type: research
Influence of dietary supplementation of vitamin A (retinol) on haematology and oxidative stress biomarkers pregnant of West African Dwarf ewes
This study investigated the influence of dietary supplementation of vitamin A (retinol) on haematology and oxidative stress biomarkers of pregnant West African dwarf ewes. A total of eighteen (18) matured ewes of averaged 15 kg were used for the experiment. The ewes were synchronized using Synchromate, a follicle stimulating hormone (FSH), at the end of 4 weeks of acclimatization to bring all eighteen (18) ewes to ovulation at the same period. The eighteen matured ewes were randomly assigned to three (3) treatment gr oups consisting of six (6) ewes per group Each group was randomly allotted to the three (3) experimental...
Source: Comparative Clinical Pathology - March 8, 2024 Category: Pathology Source Type: research
Reticular abscess in buffaloes and cattle: clinical-biochemical, diagnostic imaging findings, treatment and long-term follow-up
This study aims to describe clinical features, hemato-biochemical alterations, management, productive and reproductive performance, long term follow-up of reticular abscess in bovines. This prospective study was conducted on 12 bovines diagnosed with reticular abscess. All the animals were subjected to hematological and biochemical evaluation along with a healthy control group. The diseased animals were subjected to radiography, ultrasonography, and surgical treatment. The clinical findings were chronic anorexia, dehydration, persistent or recurrent tympany, scanty feces, mushy rumen, tachypnea, gradual reduction in milk y...
Source: Comparative Clinical Pathology - March 7, 2024 Category: Pathology Source Type: research
Phytochemistry and pharmacology of Piper nigrum
AbstractBlack pepper (Piper nigrum) has been used as an antidote for cough, cold, dyspnoea, throat illnesses, intermittent fever, dysentery, stomach ache, worms, and piles in traditional medicine. It is also used as an anti-inflammatory, antipyretic, and to treat epilepsy and snakebite. Black pepper is a common spice in Asian cuisine, and it has applications in traditional medicine, perfumes, preservatives, and pesticides. Piperine, an alkaloid, increases the therapeutic efficacy of a variety of medications, vaccines, and nutrients by increasing bioavailability by blocking several digestive enzymes. Piperine also promotes ...
Source: Comparative Clinical Pathology - March 7, 2024 Category: Pathology Source Type: research
Naringin alleviates liver damage induced by D-galactosamine (D-gal) in mice through down-regulation of CYP2E1 and P53 hyperactivity
This study aims to study the potential antioxidants and the anti-inflammatory effects of naringin in mice-induced liver damage by D-galactosamine (D-gal) administration. Mice were divided into four groups: group 1, the normal control group; group 2, the D-gal group was injected (200 mg /kg; s.c.) daily for 8 weeks; and groups 3 and 4, naringin groups received naringin (150 mg/kg and 300 mg/kg; p.o.) daily concurrent with D-gal for 8 weeks. Serum was prepared to measure aspartate transaminase (AST) and alanine transaminases (ALT). Liver tissue homogenate was prepared to me asure nitric oxide (NO), catalase, cytochrome...
Source: Comparative Clinical Pathology - February 28, 2024 Category: Pathology Source Type: research
Occupational exposure to the dust of chemical fertilizers (NPK 15.15.15): effect on biochemical parameters and oxidative stress status among workers in Annaba
AbstractThe hazardous elements contained in chemical fertilizers may not only potentially cause adverse health effects to the environment but also to professional health. The current study has been conducted to evaluate the probable effects of chemical fertilizers on occupational health. Forty male participants have been divided into two main groups, the exposed group (workers) includes 20 employees working in the fertilizer industry of Annaba (Algeria) for at least 6 years; the control group includes 20 participants with no history of exposure to any chemical fertilizer. Blood samples have been collected for biochemical, ...
Source: Comparative Clinical Pathology - February 28, 2024 Category: Pathology Source Type: research
Urtica dioica extract mitigates doxorubicin-induced hepatotoxicity and nephrotoxicity by suppressing oxidative stress and modulating biochemical indices: In vivo and molecular docking study
This study evaluated the hepatoprotective and nephroprotective effects of ethanol extracts ofUrtica dioica (UD) leaves on doxorubicin-induced oxidative stress. This study comprised 5 groups: control, doxorubicin (DOX: 15 mg/kg), UD-treated group (DOX + 300 mg/kg), a second UD-treated group (DOX + 600 mg/kg), and UD-treated group (600 mg/kg alone). The in vitro antioxidant potential of UD was as sayed with FRAP and DPPH, and GCMS-identified UD phytoconstituents were docked against NADPH oxidase (2CDU) and nitric oxide synthase (2FLQ) using molecular docking tools such as Discovery Studio, Open Babel, and PyRX...
Source: Comparative Clinical Pathology - February 20, 2024 Category: Pathology Source Type: research
Relationship of 5 α-reductase and androgens with sperm quality during breeding and non-breeding seasons in Arabi rams
AbstractSeasonal changes in the sperm quality of rams may be influenced by the activity of 5 α-reductase (5αR) and androgens, i.e., testosterone and dihydrotestosterone (DHT). The aim of this study was to investigate the variation in the levels of 5αR type 2 (5αR2) and androgens in serum and seminal plasma and their association with sperm quality parameters during the breeding season (B S) and non-breeding season (NBS) in Arabi rams. A total of ten adult pure Arabi rams with 3.5 ± 0.5 years old were used. Semen and blood samples were collected during the breeding and non-breeding seasons. Sperm quality parameters ...
Source: Comparative Clinical Pathology - February 20, 2024 Category: Pathology Source Type: research
Canine distemper with myoclonus and tonic-clonic seizures in a domestic cat (Felis catus) in Kerala, India
AbstractCanine distemper virus (CDV) is a major global pathogen of dogs affecting multiple organ systems of the body and causing the characteristic malady with prominent neurological manifestation. Natural CDV infections with typical clinical symptoms in domestic cats are not yet reported in Kerala, India, even though a few reports on the occurrence of fatal canine distemper in large felids exist. The present study reports a documented preliminary report of the natural canine distemper virus infection in a domestic cat from Kerala, India, with typical neurological manifestations. An unvaccinated adult female domestic cat w...
Source: Comparative Clinical Pathology - February 13, 2024 Category: Pathology Source Type: research
Evaluation of the effect of Dracocephalum kotschyi hydroalcoholic extract in preventing intra-abdominal adhesions after abdominal surgery in rats based on inflammatory factors (TNF- α and IL-1β)
In this study, we investigated the anti-abdominal adhesions effect of the hydroalcoholic extract ofDracocephalum kotschyi. It is an experimental study. Forty healthy male Wistar rats were divided into 6 groups and abdominal surgery was performed in all of them to induce adhesion: group 1, control (with surgery and without active ingredient); group 2, chitosan gel injection in the abdominal area before suturing the site (as anti-adhesive); group 3, administration of alcoholic extract ofDracocephalum kotschyi at a dose of 40 mg/kg; group 4, administration of alcoholic extract ofDracocephalum kotschyi at a dose of 80 mg/kg; g...
Source: Comparative Clinical Pathology - February 12, 2024 Category: Pathology Source Type: research
The therapeutic potential of saharian monovarietal virgin olive oil (olea europea l.) on hematological, histological, and antioxidant status in titanium dioxide nanoparticles-induced oxidative stress in rats
This study aimed to assess the composition of bioactive compounds and the antioxidant activity of monovarietal virgin olive oil (MVOO). Specifically, the objective was to investigate the protective effects of MVOO in alleviating the adverse effects induced by titanium dioxide nanoparticles (NPTiO2). Bioactive compounds in MVOO were analyzed using UV –visible spectrophotometry, along with High-Performance Liquid Chromatography with a Photodiode Array (HPLC–PDA) for a comprehensive assessment. Antioxidant activity in vitro was evaluated through 2,2-diphenyl-1-picrylhydrazyl (DDPH) assays and on rats in vivo. A total of t...
Source: Comparative Clinical Pathology - February 7, 2024 Category: Pathology Source Type: research
Gene expression of hemostasis biomarkers following HIIT and RIPC
The objective of this study was to examine how these strategies might affect gene expression of hemostasis biomarkers in middle-aged male Wistar rats. Twenty-four male Wistar rats (12-month-old) were randomly assigned to four groups: high-intensity interval training (HIIT), remote ischemic preconditioning +HIIT (RIPC+HIIT), sham-HIIT (S-HIIT), sham-RIPC+HIIT (S-RIPC+HIIT). The experimental groups followed specific exercise protocols three times per week. The HIIT involved a 5-min warm-up period at 40–50% vVO2max, followed by 6 sets of 2-min high-intensity exercise at 85 –90% vVO2max, interspersed by 5-min inactive r...
Source: Comparative Clinical Pathology - February 6, 2024 Category: Pathology Source Type: research
Modulation of PON-1 by acetate reverses hepatic dysmetabolism in experimentally induced PCOS
AbstractPolycystic ovarian syndrome (PCOS) is well documented as the leading cause of infertility globally, and its long-term complications include non-alcoholic fatty liver disease (NAFLD) which results in a cascade of events leading to liver cirrhosis as well as hepatic carcinoma. Paraoxonase-1 (PON-1) is an antiatherosclerotic component, which protects against hepatic injury. Short-chain fatty acids (SCFAs), particularly acetate, are synthesized by the gut microbiota and play a central role in metabolic regulation. The present study investigated the impact of acetate on PCOS-associated hepatic dysregulation and probable...
Source: Comparative Clinical Pathology - February 6, 2024 Category: Pathology Source Type: research
