Co-Immobilization of ADH and GDH on Metal-organic-framework: An Effective Biocatalyst for Asymmetric Reduction of Ketones
Chembiochem. 2024 Apr 17:e202400147. doi: 10.1002/cbic.202400147. Online ahead of print.ABSTRACTChiral alcohols are not only important building blocks of various bioactive natural compounds and pharmaceuticals, but can serve as synthetic precursors for other valuable organic chemicals, thus the synthesis of these products is of great importance. Bio-catalysis represents one effective way to obtain these molecules, however, the weak stability and high cost of enzymes often hinder its broad application. In this work, we designed a biological nanoreactor by embedding alcohol dehydrogenase (ADH) and glucose dehydrogenase (GDH)...
Source: Chembiochem - April 17, 2024 Category: Biochemistry Authors: Lu Ran Guorong Su Zhenyan Yang Shuke Wu Huailong Teng Source Type: research
Monomeric far-red and near-infrared fluorescent biliproteins of ultrahigh brightness
We report that fluorescent phycobiliproteins originating from the phycobilisome core subunit ApcF2 can covalently bind biliverdin, named BDFPs. To further improve BDFPs, we conducted a series of studies. Firstly, we mutated K53Q and T144A of BDFPs to increase their effective brightness up to 190% in vivo. Secondly, by homochromatic tandem fusion of high-brightness BDFPs to achieve monomerization, which increases the effective brightness by up to 180% in vivo, and can effectively improve the labeling effect. By combining the above two approaches, the brightness of the tandem BDFPs was much improved compared with that of the...
Source: Chembiochem - April 16, 2024 Category: Biochemistry Authors: Xiang-Xiang Jiang Ya-Nan Hou Li-Wen Lu Kai-Hong Zhao Source Type: research
Gold Nanoparticle Delivery of Glut1 siRNA Facilitates Glucose Starvation Therapy in Lung Cancer
Chembiochem. 2024 Apr 16:e202400239. doi: 10.1002/cbic.202400239. Online ahead of print.ABSTRACTGlucose transporter protein-1 (Glut1), is highly expressed in many cancer types and plays a crucial role in cancer progression through enhanced glucose transport. Its overexpression is associated with aggressive tumor behavior and poor prognosis. Herein, the nucleic acids modified gold nanoparticles (AuNPs) was synthesized to deliver small interfering RNA (siRNA) against Glut1 by microRNA 21 (miR-21) triggers toehold-mediated strand displacement reaction for lung cancer starvation therapy. Overexpression of miR-21 triggers toeho...
Source: Chembiochem - April 16, 2024 Category: Biochemistry Authors: Jiaqi Li Jingyuan Yu Qi Fang Yan Du Xiaojun Zhang Source Type: research
Wall-immobilized biocatalyst vs. packed bed in miniaturized continuous reactors: performances and scale-up
Chembiochem. 2024 Apr 15:e202400086. doi: 10.1002/cbic.202400086. Online ahead of print.ABSTRACTSustainable biocatalysis syntheses have gained considerable popularity over the years. However, further optimizations - notably to reduce costs - are required if the methods are to be successfully deployed in a range of areas. As part of this drive, various enzyme immobilization strategies have been studied, alongside process intensification from batch to continuous production. The flow bioreactor portfolio mainly ranges between packed bed reactors and wall-immobilized enzyme miniaturized reactors. Because of their simplicity, p...
Source: Chembiochem - April 15, 2024 Category: Biochemistry Authors: Maite Michaud Guillaume Nonglaton Zo é Anxionnaz-Minvielle Source Type: research
Systematic evaluation of benchmark G4 probes and G4 clinical drugs using three biophysical methods: A guideline to evaluate rapidly G4-binding affinity
Chembiochem. 2024 Apr 15:e202400210. doi: 10.1002/cbic.202400210. Online ahead of print.ABSTRACTG-quadruplex DNA structures (G4) are proven to interfere with most genetic and epigenetic processes. Small molecules binding these structures (G4 ligands) are invaluable tools to probe G4-biology and address G4-druggability in various diseases (cancer, viral infections). However, the large number of reported G4 ligands (> 1000) could lead to confusion while selecting one for a given application. Herein we conducted a systematic affinity ranking of 11 popular G4 ligands vs 5 classical G4 sequences using FRET-melting, G4-FID as...
Source: Chembiochem - April 15, 2024 Category: Biochemistry Authors: Eugenie Laigre Hugues Bonnet Claire Beauvineau Thomas Lavergne Daniela Verga Eric Defrancq Jerome Dejeu Marie-Paule Teulade-Fichou Source Type: research
Identifying Potential SOS1 Inhibitors via Virtual Screening of Multiple Small Molecule Libraries against KRAS-SOS1 Interface
In this study, advanced in silico techniques were employed to screen small molecule libraries at this interface, leading to the identification of promising lead compounds as potential SOS1 inhibitors. Comparative analysis of the average binding free energies of these predicted potent compounds with known SOS1 small molecule inhibitors revealed that the identified compounds display similar or even superior predicted binding affinities compared to the known inhibitors. These findings offer valuable insights into the potential of these compounds as candidates for further development as effective anti-cancer agents.PMID:386220...
Source: Chembiochem - April 15, 2024 Category: Biochemistry Authors: Saima Ikram Ehsan Sayyah Serdar Durdagi Source Type: research
Systematic analysis of the effect of genomic knock-out of non-essential promiscuous HAD-like phosphatases YcsE, YitU and YwtE on flavin and adenylate content in Bacillus subtilis
Chembiochem. 2024 Apr 14:e202400165. doi: 10.1002/cbic.202400165. Online ahead of print.ABSTRACTStudying the metabolic role of non-essential promiscuous enzymes is a challenging task, as genetic manipulations usually do not reveal at which point(s) of the metabolic network the enzymatic activity of such protein is beneficial for the organism. Each of the HAD-like phosphatases YcsE, YitU and YwtE of Bacillus subtilis catalyzes the dephosphorylation of 5-amino-6-ribitylamino-uracil 5'-phosphate, which is essential in the biosynthesis of riboflavin. Using CRISPR technology, we have found that the deletion of these genes, indi...
Source: Chembiochem - April 14, 2024 Category: Biochemistry Authors: Alexandra Scharf Farshad La-Rostami Boris A Illarionov Vivien Nemes Anna M Feldmann Lars S H öft Henri L ösel Adelbert Bacher Markus Fischer Source Type: research
Peptide-Based Recognition Agents of Histamine: A Biopanning Approach with Enhanced Specificity
In this study, we identified histamine-binding peptides by screening a random 12-mer peptide library, employing a novel biopanning approach that excluded histidine-binding sequences in the final round. This additional step enhanced the selectivity of the peptides and prevented interference from histidine during detection. The binding affinities of synthesized peptides to histamine were assessed using isothermal titration calorimetry (ITC). Among the identified peptides, HBF10 (SGFRDGIEDFLW) and HBF26 (IPLENQHKIYST) showed significant affinity to histamine, with Kavalues of 2.56×104 (M-1) and 8.94×104 (M-1), respectively....
Source: Chembiochem - April 14, 2024 Category: Biochemistry Authors: Hafize Öz Fahriye Ceyda Dudak Source Type: research
Utilization of Free and Dipeptide-Bound Formyline and Pyrraline by Saccharomyces Yeasts
Chembiochem. 2024 Apr 13:e202300854. doi: 10.1002/cbic.202300854. Online ahead of print.ABSTRACTThe utilization of the glycated amino acids formyline and pyrraline and their peptide-bound derivatives by 14 Saccharomyces yeasts, including 6 beer yeasts (bottom and top fermenting), one wine yeast, 6 strains isolated from natural habitats and one laboratory reference yeast strain (wild type) was investigated. All yeasts were able to metabolize glycated amino acids via the Ehrlich pathway to the corresponding Ehrlich metabolites. While formyline and small amounts of pyrraline entered the yeast cells via passive diffusion, the ...
Source: Chembiochem - April 13, 2024 Category: Biochemistry Authors: Anna-Lena Kertsch Jana Einicke Julia Miedl Michael Hellwig Thomas Henle Source Type: research
SARS-CoV-2 Spike Protein-Derived Cyclic Peptides as Modulators of Spike Interaction with GRP78
Chembiochem. 2024 Apr 13:e202300789. doi: 10.1002/cbic.202300789. Online ahead of print.ABSTRACTThe human glucose-regulated protein GRP78 is a human chaperone that translocactes to the cell surface when cells are under stress. Theoretical studies suggested it could be involved in SARS-CoV-2 virus entry to cells. In this work, we used in vitro surface plasmon resonance-based assays to show that human GRP78 indeed binds to SARS-CoV-2 spike protein. We have designed and synthesised cyclic peptides based on the loop structure of amino acids 480-488 of the SARS-CoV-2 spike protein S1 domain from the Wuhan and Omicron variants a...
Source: Chembiochem - April 13, 2024 Category: Biochemistry Authors: Nicholas Johnson Craig Pattinson Kate Burgoyne Karolin Hijazi Wael E Houssen Bruce Forbes Milne Source Type: research
Chemistry of Strigolactones, Key Players in Plant Communication
We present in this review the diversity of natural SLs, their analogs, mimics, and probes essential for the biological studies of this class of compounds. Their biosynthesis and access by organic synthesis are highlighted especially concerning noncanonical SLs, the more recently discovered natural SLs. Organic synthesis of analogs, stable isotope-labeled standards, mimics, and probes are also reviewed here. In the last part, the knowledge about the SL perception is described as well as the different inhibitors of SL receptors that have been developed.PMID:38607659 | DOI:10.1002/cbic.202400133 (Source: Chembiochem)
Source: Chembiochem - April 12, 2024 Category: Biochemistry Authors: Suzanne Daignan-Fornier Antoinette Keita Francois-Didier Boyer Source Type: research
AM404 Analogs as Activators of the 20S Isoform of the Human Proteasome
Chembiochem. 2024 Apr 12:e202400284. doi: 10.1002/cbic.202400284. Online ahead of print.ABSTRACTThe proteasome is a multisubunit protease system responsible for the majority of the protein turnover in eukaryotic organisms. Dysregulation of this enzymatic complex leads to protein accumulation, subsequent aggregation, and ultimately diseased states; for that reason, positive modulation of its activity has been recently investigated as a therapeutic strategy for neurodegenerative and age-related diseases. The small molecule AM404 was recently identified as an activator of the 20S isoform of the proteasome and further explorat...
Source: Chembiochem - April 12, 2024 Category: Biochemistry Authors: Andres Salazar Kate Kragness Diogo Feleciano Darci Jones Trader Source Type: research
Chemistry of Strigolactones, Key Players in Plant Communication
We present in this review the diversity of natural SLs, their analogs, mimics, and probes essential for the biological studies of this class of compounds. Their biosynthesis and access by organic synthesis are highlighted especially concerning noncanonical SLs, the more recently discovered natural SLs. Organic synthesis of analogs, stable isotope-labeled standards, mimics, and probes are also reviewed here. In the last part, the knowledge about the SL perception is described as well as the different inhibitors of SL receptors that have been developed.PMID:38607659 | DOI:10.1002/cbic.202400133 (Source: Chembiochem)
Source: Chembiochem - April 12, 2024 Category: Biochemistry Authors: Suzanne Daignan-Fornier Antoinette Keita Francois-Didier Boyer Source Type: research
Why Does Monoamine Oxidase (MAO) Catalyze the Oxidation of Some Tetrahydropyridines?
Chembiochem. 2024 Apr 11:e202400126. doi: 10.1002/cbic.202400126. Online ahead of print.ABSTRACTResults pertaining to the mechanism of the oxidation of the tertiary amine 1-methyl-4-(1-methyl-1-H-pyrrol-2-yl)-1,2,3,6-tetrahydropyridine (MMTP, a close analog of the Parkinsonism inducing compound MPTP) by 3-methyllumiflavin (3MLF), a chemical model for the FAD cofactor of monoamine oxidase, are reported. MMTP and related compounds are among the few tertiary amines that are monoamine oxidase B (MAO-B) substrates. The MMTP/3MLF reaction is catalytic in the presence of O2 and the results under anaerobic conditions strongly sugg...
Source: Chembiochem - April 11, 2024 Category: Biochemistry Authors: Nathan J Price Akiko Nakamura Neal Castagnoli James M Tanko Source Type: research
The PR-10 protein Pru p 1 is an endonuclease that preferentially cleaves single-stranded RNA
In this study, we elucidate the enzymatic activity of Pru p 1, a PR-10 protein from peach. We demonstrate that this protein catalyzes the endonucleolytic backbone cleavage of RNA substrates into short oligonucleotides. Initial cleavage products, identified through kinetic analysis, can bind again, priming them for further degradation. NMR binding site mapping reveals that the large internal cavity of Pru p 1, which is characteristic for PR-10 proteins, serves as an anchoring site for single-stranded ribonucleotide chains. We propose a structure-based mechanistic model that accounts for the observed cleavage patterns and th...
Source: Chembiochem - April 11, 2024 Category: Biochemistry Authors: Manuel R öck Sarah Viola Heel Fabian Sebastian Juen Reiner Eidelpes Christoph Kreutz Kathrin Breuker Martin Tollinger Source Type: research
