Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice
Am J Physiol Cell Physiol. 2024 Apr 1;326(4):C1272-C1290. doi: 10.1152/ajpcell.00446.2023. Epub 2024 Mar 4.ABSTRACTSodium-glucose cotransporter, type 2 inhibitors (SGLT2i) are emerging as the gold standard for treatment of type 2 diabetes (T2D) with renal protective benefits independent of glucose lowering. We took a high-level approach to evaluate the effects of the SGLT2i, empagliflozin (EMPA) on renal metabolism and function in a prediabetic model of metabolic syndrome. Male and female 12-wk-old TallyHo (TH) mice, and their closest genetic lean strain (Swiss-Webster, SW) were treated with a high-milk-fat diet (HMFD) plu...
Source: Am J Physiol Cell Ph... - April 11, 2024 Category: Cytology Authors: Blythe D Shepard Jennifer Chau Ryan Kurtz Avi Z Rosenberg Pinaki Sarder Samuel P Border Brandon Ginley Olga Rodriguez Chris Albanese Grace Knoer Aarenee Greene Aline M A De Souza Suman Ranjit Moshe Levi Carolyn M Ecelbarger Source Type: research
Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice
Am J Physiol Cell Physiol. 2024 Apr 1;326(4):C1272-C1290. doi: 10.1152/ajpcell.00446.2023. Epub 2024 Mar 4.ABSTRACTSodium-glucose cotransporter, type 2 inhibitors (SGLT2i) are emerging as the gold standard for treatment of type 2 diabetes (T2D) with renal protective benefits independent of glucose lowering. We took a high-level approach to evaluate the effects of the SGLT2i, empagliflozin (EMPA) on renal metabolism and function in a prediabetic model of metabolic syndrome. Male and female 12-wk-old TallyHo (TH) mice, and their closest genetic lean strain (Swiss-Webster, SW) were treated with a high-milk-fat diet (HMFD) plu...
Source: Am J Physiol Cell Ph... - April 11, 2024 Category: Cytology Authors: Blythe D Shepard Jennifer Chau Ryan Kurtz Avi Z Rosenberg Pinaki Sarder Samuel P Border Brandon Ginley Olga Rodriguez Chris Albanese Grace Knoer Aarenee Greene Aline M A De Souza Suman Ranjit Moshe Levi Carolyn M Ecelbarger Source Type: research
SIRT4 promotes neuronal apoptosis in models of Alzheimer's disease via the STAT2-SIRT4-mTOR pathway
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00012.2024. Online ahead of print.ABSTRACTAlzheimer's disease (AD) is the leading cause of dementia and presents a considerable disease burden. Its pathology involves substantial neuronal loss, primarily attributed to neuronal apoptosis. Although sirtuin 4 (SIRT4) has been implicated in regulating apoptosis in various diseases, the role of SIRT4 in AD pathology remains unclear. The study employed APP/PS1 mice as an animal model of AD and amyloid-β (Aβ)1-42-treated HT-22 cells as an AD cell model. SIRT4 expression was determined by quantitative real-time polymera...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Dianxia Xing Wenjin Zhang Wei Cui Xiuya Yao Yaping Xiao Lihua Chen Shiyun Yuan Yanyan Duan Weihua Yu Pengfei Pan Yang L ü Source Type: research
Time-of-day effects on < em > ex vivo < /em > muscle contractility following short-term satellite cell ablation
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00157.2024. Online ahead of print.ABSTRACTMuscle isometric torque fluctuates according to time-of-day with such variation owed to the influence of circadian molecular clock genes. Satellite cells (SC), the muscle stem cell population, also express molecular clock genes with several contractile related genes oscillating in a diurnal pattern. Currently, limited evidence exists regarding the relationship between SCs and contractility, although long-term SC ablation alters muscle contractile function. Whether there are acute alterations in contractility following SC a...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Ryan Edward Kahn Richard L Lieber Guadalupe Meza Fawzan Dinnunhan Orly Lacham-Kaplan Sudarshan Dayanidhi John A Hawley Source Type: research
Visceral adipose of obese mice inhibits endothelial inwardly rectifying K < sup > + < /sup > channels in a CD36-dependent fashion
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00073.2024. Online ahead of print.ABSTRACTObesity imposes deficits to adipose tissue and vascular endothelium, yet the role that distinct adipose depots play in mediating endothelial dysfunction in local arteries remains unresolved. We recently showed that obesity impairs endothelial Kir2.1 channels, mediators of NO production, in arteries of visceral adipose tissue (VAT) while Kir2.1 function in subcutaneous adipose tissue (SAT) endothelium remains intact. Therefore, we determined if VAT vs. SAT from lean or diet-induced obese mice affected Kir2.1 channel functio...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Sabita Rokka Masoumeh Sadeghinejad Emma C Hudgins Erica J Johnson Thanh Nguyen Ibra S Fancher Source Type: research
Metformin Mitigates Adipogenesis of Fibro-adipogenic Progenitors After Rotator Cuff Tears Via Activating mTOR/ULK1 Mediated Autophagy
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00034.2024. Online ahead of print.ABSTRACTMuscular fatty infiltration is a common issue after rotator cuff tears (RCT) which impairs shoulder function. Females suffer higher prevalence and more severe degree of muscular fatty infiltration after RCT when compared to males, with the underlying mechanisms remaining unclear. Fibro/adipogenic progenitors (FAPs) are the primary source of muscular fatty infiltration following RCT. Our findings disclose that gender-specific disparities in muscular fatty infiltration are linked to mTOR/ULK1-mediated autophagy of FAPs. Decr...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Hao Zhou Xingzuan Lin Shujing Feng Siyuan Zhu Han Zhou Huifang Chen He Youwu Zekai Wang Ru Wang Xiexiang Shao Jianhua Wang Source Type: research
Ox-LDL-induced CD80 < sup > + < /sup > macrophages expand pro-atherosclerotic NKT cells via CD1d in atherosclerotic mice and hyperlipidemic patients
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00043.2024. Online ahead of print.ABSTRACTAtherosclerosis is an inflammatory disease of blood vessels involving the immune system. Natural killer T (NKT) cells, as crucial components of the innate and acquired immune systems, play critical roles in the development of atherosclerosis. However, the mechanism and clinical relevance of NKT cells in early atherosclerosis are largely unclear. Our findings demonstrated that there were higher populations of NKT cells and interferon gamma (IFN-γ)-producing NKT cells in the peripheral blood of patients with hyperlipidemia ...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Yin Wang Yao Zou Qingsong Jiang Wenming Li Xinyu Chai Tingrui Zhao Siyi Liu Zhiyi Yuan Chao Yu Tingting Wang Source Type: research
SIRT4 promotes neuronal apoptosis in models of Alzheimer's disease via the STAT2-SIRT4-mTOR pathway
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00012.2024. Online ahead of print.ABSTRACTAlzheimer's disease (AD) is the leading cause of dementia and presents a considerable disease burden. Its pathology involves substantial neuronal loss, primarily attributed to neuronal apoptosis. Although sirtuin 4 (SIRT4) has been implicated in regulating apoptosis in various diseases, the role of SIRT4 in AD pathology remains unclear. The study employed APP/PS1 mice as an animal model of AD and amyloid-β (Aβ)1-42-treated HT-22 cells as an AD cell model. SIRT4 expression was determined by quantitative real-time polymera...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Dianxia Xing Wenjin Zhang Wei Cui Xiuya Yao Yaping Xiao Lihua Chen Shiyun Yuan Yanyan Duan Weihua Yu Pengfei Pan Yang L ü Source Type: research
Time-of-day effects on < em > ex vivo < /em > muscle contractility following short-term satellite cell ablation
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00157.2024. Online ahead of print.ABSTRACTMuscle isometric torque fluctuates according to time-of-day with such variation owed to the influence of circadian molecular clock genes. Satellite cells (SC), the muscle stem cell population, also express molecular clock genes with several contractile related genes oscillating in a diurnal pattern. Currently, limited evidence exists regarding the relationship between SCs and contractility, although long-term SC ablation alters muscle contractile function. Whether there are acute alterations in contractility following SC a...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Ryan Edward Kahn Richard L Lieber Guadalupe Meza Fawzan Dinnunhan Orly Lacham-Kaplan Sudarshan Dayanidhi John A Hawley Source Type: research
Visceral adipose of obese mice inhibits endothelial inwardly rectifying K < sup > + < /sup > channels in a CD36-dependent fashion
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00073.2024. Online ahead of print.ABSTRACTObesity imposes deficits to adipose tissue and vascular endothelium, yet the role that distinct adipose depots play in mediating endothelial dysfunction in local arteries remains unresolved. We recently showed that obesity impairs endothelial Kir2.1 channels, mediators of NO production, in arteries of visceral adipose tissue (VAT) while Kir2.1 function in subcutaneous adipose tissue (SAT) endothelium remains intact. Therefore, we determined if VAT vs. SAT from lean or diet-induced obese mice affected Kir2.1 channel functio...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Sabita Rokka Masoumeh Sadeghinejad Emma C Hudgins Erica J Johnson Thanh Nguyen Ibra S Fancher Source Type: research
Metformin Mitigates Adipogenesis of Fibro-adipogenic Progenitors After Rotator Cuff Tears Via Activating mTOR/ULK1 Mediated Autophagy
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00034.2024. Online ahead of print.ABSTRACTMuscular fatty infiltration is a common issue after rotator cuff tears (RCT) which impairs shoulder function. Females suffer higher prevalence and more severe degree of muscular fatty infiltration after RCT when compared to males, with the underlying mechanisms remaining unclear. Fibro/adipogenic progenitors (FAPs) are the primary source of muscular fatty infiltration following RCT. Our findings disclose that gender-specific disparities in muscular fatty infiltration are linked to mTOR/ULK1-mediated autophagy of FAPs. Decr...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Hao Zhou Xingzuan Lin Shujing Feng Siyuan Zhu Han Zhou Huifang Chen He Youwu Zekai Wang Ru Wang Xiexiang Shao Jianhua Wang Source Type: research
Ox-LDL-induced CD80 < sup > + < /sup > macrophages expand pro-atherosclerotic NKT cells via CD1d in atherosclerotic mice and hyperlipidemic patients
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00043.2024. Online ahead of print.ABSTRACTAtherosclerosis is an inflammatory disease of blood vessels involving the immune system. Natural killer T (NKT) cells, as crucial components of the innate and acquired immune systems, play critical roles in the development of atherosclerosis. However, the mechanism and clinical relevance of NKT cells in early atherosclerosis are largely unclear. Our findings demonstrated that there were higher populations of NKT cells and interferon gamma (IFN-γ)-producing NKT cells in the peripheral blood of patients with hyperlipidemia ...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Yin Wang Yao Zou Qingsong Jiang Wenming Li Xinyu Chai Tingrui Zhao Siyi Liu Zhiyi Yuan Chao Yu Tingting Wang Source Type: research
SIRT4 promotes neuronal apoptosis in models of Alzheimer's disease via the STAT2-SIRT4-mTOR pathway
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00012.2024. Online ahead of print.ABSTRACTAlzheimer's disease (AD) is the leading cause of dementia and presents a considerable disease burden. Its pathology involves substantial neuronal loss, primarily attributed to neuronal apoptosis. Although sirtuin 4 (SIRT4) has been implicated in regulating apoptosis in various diseases, the role of SIRT4 in AD pathology remains unclear. The study employed APP/PS1 mice as an animal model of AD and amyloid-β (Aβ)1-42-treated HT-22 cells as an AD cell model. SIRT4 expression was determined by quantitative real-time polymera...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Dianxia Xing Wenjin Zhang Wei Cui Xiuya Yao Yaping Xiao Lihua Chen Shiyun Yuan Yanyan Duan Weihua Yu Pengfei Pan Yang L ü Source Type: research
Time-of-day effects on < em > ex vivo < /em > muscle contractility following short-term satellite cell ablation
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00157.2024. Online ahead of print.ABSTRACTMuscle isometric torque fluctuates according to time-of-day with such variation owed to the influence of circadian molecular clock genes. Satellite cells (SC), the muscle stem cell population, also express molecular clock genes with several contractile related genes oscillating in a diurnal pattern. Currently, limited evidence exists regarding the relationship between SCs and contractility, although long-term SC ablation alters muscle contractile function. Whether there are acute alterations in contractility following SC a...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Ryan Edward Kahn Richard L Lieber Guadalupe Meza Fawzan Dinnunhan Orly Lacham-Kaplan Sudarshan Dayanidhi John A Hawley Source Type: research
Visceral adipose of obese mice inhibits endothelial inwardly rectifying K < sup > + < /sup > channels in a CD36-dependent fashion
Am J Physiol Cell Physiol. 2024 Apr 8. doi: 10.1152/ajpcell.00073.2024. Online ahead of print.ABSTRACTObesity imposes deficits to adipose tissue and vascular endothelium, yet the role that distinct adipose depots play in mediating endothelial dysfunction in local arteries remains unresolved. We recently showed that obesity impairs endothelial Kir2.1 channels, mediators of NO production, in arteries of visceral adipose tissue (VAT) while Kir2.1 function in subcutaneous adipose tissue (SAT) endothelium remains intact. Therefore, we determined if VAT vs. SAT from lean or diet-induced obese mice affected Kir2.1 channel functio...
Source: Am J Physiol Cell Ph... - April 8, 2024 Category: Cytology Authors: Sabita Rokka Masoumeh Sadeghinejad Emma C Hudgins Erica J Johnson Thanh Nguyen Ibra S Fancher Source Type: research
